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Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Article Title: Interleukin 15-Presenting Nanovesicles with Doxorubicin-Loaded Ferritin Cores for Cancer Immunochemotherapy.
doi: 10.1002/advs.202409194
Figure Lengend Snippet: Figure 2. Association between intratumoral transcription of IL15 and IL2RG and survival in TNBC patients and the effect of IL15 and IL15c on tumor growth and cell proliferation. a) Kaplan–Meier analysis of the overall survival (OS) probability of two cohorts TNBC patients (Sabatier: 83 patients; METABRIC: 216 patients) with different transcription levels of intratumoral IL2RG and IL15 genes. Statistical analysis was calculated by a two-sided log-rank test. b,c) The tumor growth profiles of 4T1 tumor-bearing mice following the indicated treatments. Treatments were initiated when the tumor volumes reached ≈100 mm3. IL15 (b) or IL15c (c) were dosed intratumorally (0.04 nmol per mouse at day 0, day 4, and day 8). Data were presented as mean ± s.d. (n = 6 biologically independent animals). Statistical significance was calculated by a two-way ANOVA test. d,e) The growth profiles of CTLL-2 (d) or 4T1 (e) cells following the indicated treatments in 72 h. 4T1 or CTLL-2 cells (2 × 104 cells) were incubated in 8 mL 1640 medium in the presence or absence of either IL15c or IL15 (0.2 nm). The number of live cells was determined using a cell counter after Trypan Blue (0.4%) staining. Data were presented as mean ± s.d. (n = 3 biological replicates per group). Statistical significance was calculated by a two-way ANOVA test. f,g) 4T1 cells were pretreated by IFN-𝛾(1 ng mL−1) for over 12 h. The growth profiles of 4T1 cells (2 × 104 cells in 8 mL medium) after exposure to IL15 or IL15c (0.2 nm) at the presence of PBS (f) or antibodies (g). (n = 3 biologically independent samples).
Article Snippet: The primary antibodies used for Western blot were against IL15Rα (R&D, AF551),
Techniques: Incubation, Staining